Amazonocrinis thailandica sp. nov. (Nostocales, Cyanobacteria), a novel species of the previously monotypic Amazonocrinis genus from Thailand

Cyanobacteria are distributed worldwide, and many new cyanobacterial species discovered in tropical region. TheNostoc-like genus Amazonocrinis has been separated from the Nostoc based on polyphasic methods. However,species diversity within this remains poorly understood systematically because only one (Amazonocrinisnigriterrae) described. In study, two novel strains (NUACC02 NUACC03) were isolated moist ricefield soil Thailand. These characterized using a approach, morphology, 16S rRNAphylogenetic analysis, internal transcribed spacer secondary structure ecology. Phylogenetic analyses 16SrRNA gene sequences confirmed that formed monophyletic clade related to Amazonocrinisand distant type A. nigriterrae. The rRNA sequence similarity (<98.1%) betweennovel all other closely taxa including members exceeded cutoff for speciesdelimitation bacteriology, reinforcing presence of species. Furthermore, strainspossessed unique phenotypic characteristics such as sheath, necridia-like cells, larger cell dimensionand akinete arrangement long-chains singularity D1–D1′, Box-B, V2, V3 structures thatdistinguished them members. Considering results, we described our Amazonocrinisthailandica sp. nov. accordance with International Code Nomenclature Algae, Fungi Plants.